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KMID : 1161420110140060664
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Journal of Medicinal Food
2011 Volume.14 No. 6 p.664 ~ p.669
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Assessment of Phenolic Acid Content and In Vitro Antiradical Characteristics of Hawthorn
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Ozturk Nilgun
Tuncel Muzaffer
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Abstract
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The infusions and extracts obtained from leaves with flowers, fruit peel, and seed from hawthorn (Crataegus monogyna Jacq., Family Rosaceae) were subjected to evaluation as potential sources of antioxidant phytochemicals on the basis of their total content of phenolics, levels of phenolic acids, and in vitro antiradical activity. Total phenolic content of extracts was determined using the modified Folin?Ciocalteau method. Antioxidant activity was determined for phenolic extracts by a method involving the use of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). Phenolic acids containing extracts and infusions from hawthorn leaves, fruit peel, and seeds were obtained using different polarity solvents and separated by reverse-phase high-performance liquid chromatography, which enabled improved separation by the use of a C18 column, an acidic mobile phase, and gradient elusion. The highest total phenolic content (343.54?mg of gallic acid equivalents/g) and the highest DPPH radical scavenging activity as the inhibition percentage (60.36%) were obtained in ethyl acetate extract from hawthorn leaves with flower. Also, the highest phenolic acid content was measured in the extracts of hawthorn leaves with flowers: protocathechuic (108?128?mg/100?g), p-hydroxy benzoic (141?468?mg/100?g), caffeic (137?3,580?mg/100?g), chlorogenic (925?4,637?mg/100?g), ferulic (3,363?3,462?mg/100?g), vanillic (214?mg/100?g), and syringic (126?mg/100?g) acids. The results indicate that hawthorn is a promising plant because of its high antioxidant activity.
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KEYWORD
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antiradical activity, Crataegus monogyna Jacq., hawthorn, phenolic acids, reversed-phase high-performance liquid chromatography
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